Easily and efficiently isolate highly purified mouse pan-B cells (CD19+, CD19+CD138+, and CD138+), including conventional B-2 B cells, B-1 B cells and plasma cells from single-cell suspensions of splenocytes or other tissues samples by immunomagnetic negative selection, with the EasySep™ Mouse Pan-B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD4, CD8, CD11c, CD49b, CD90.2, Ly-6C/G (Gr-1) and TER119. The magnetically labeled cells are then separated from the untouched desired pan-B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 10 minutes, the desired pan-B cells are ready for downstream applications such as flow cytometry, culture, or cell-based assays.
This kit is compatible for use with cells from disease models where the malignant cells (B-CLL) express CD43 or CD11b. For isolation of conventional B cells only, we recommend using the EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.