Easily and efficiently isolate highly purified mouse CD4+ T cells from single-cell suspensions of splenocytes or other tissues by immunomagnetic negative selection, with the EasySep™ Mouse CD4+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD11b, CD45R, Ter119, CD8a, CD49b, CD19, CD11c, TCRgd and CD24. The magnetically labeled cells are then separated from the untouched desired mouse CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 15 minutes, the desired CD4+ T cells are ready for downstream applications such as s flow cytometry, culture, and cell-based experiments.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and mor