Easily and efficiently isolate highly purified mouse natural killer (NK) cells (CD3-CD49b+) from single-cell suspensions of mouse splenocytes and other tissue samples by immunomagnetic negative selection, with the EasySep™ Mouse NK Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD4, CD5, Ly6G, CD8a, F4/80, CD3e, CD19, CD24, TCRgd, and 7-4. The magnetically labeled cells are then separated from the untouched desired NK cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 25 minutes, the desired NK cells are ready for downstream applications such as flow cytometry, culture, or cell-based assays.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.