Isolate highly purified human CD8+ T cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human CD8 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD8 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation in as little as 15 minutes, the desired CD8+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.
This product replaces the EasySep™ Human CD8 Positive Selection Kit (Catalog #18053) for even faster cell isolations.
For large-scale isolation of human CD8+ cells from leukapheresis samples, see the large-format (1x1010 cells) kit (Catalog #100-0699).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD14+ Monocytes, Frozen isolated with EasySep™ Human CD8 Positive Selection Kit II. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.