Easily and efficiently isolate highly purified human CD14+CD16- monocytes from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Monocyte Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, dendritic cells, NK cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired CD14+CD16- monocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 12.5 minutes, the desired monocytes are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This product replaces EasySep™ Human Monocyte Enrichment Kit (Catalog #19059) for even faster cell isolations with reduced platelet contamination.
For large-scale isolation of CD14+CD16- monocytes from leukapheresis samples, see the large-format (1x1010 cells) kit (Catalog #100-0697).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood Monocytes, Fresh isolated with EasySep™ Human Monocyte Isolation Kit. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.