产地 | 美国 |
保存条件 | 2-8° |
品牌 | biolife |
货号 | 210502 |
用途 | 细胞培养 |
组织来源 | 无 |
细胞形态 | 好 |
是否是肿瘤细胞 | 否 |
保质期 | 2年 |
器官来源 | 无 |
免疫类型 | 无 |
品系 | 无 |
生长状态 | 好 |
物种来源 | 无 |
包装规格 | 100ml |
是否进口 | 是 |
For use in the setup and verification of the doublet discrimination function of FACSCalibur™, FACSort™, or FACScan™ flow cytometers equipped with a doublet discrimination module (DDM) for DNA analysis, and for monitoring daily instrument performance. For use with other FACS® brand flow cytometers, all performance specifications will need to be determined for each individual case although the particles will meet the stability criteria as stated.
Contents: Chicken erythrocyte nuclei (CEN)| calf thymocyte nuclei (CTN)| instrument QC beads| propidium iodide solution.
1. When stored at 2° to 8°C, the reagent is stable until the expiration date shown on the label. Do not use after the expiration date. Keep the reagent vials dry.
2. There is a tendency for the CEN (vial A) to settle during storage. This can be corrected by gently vortexing before use. Vortexing too vigorously will separate desired aggregates. It is critical that CEN be stored at 2° to 8°C, since these particles are sensitive to thermal degradation.
3. CTN (vial B) tend to aggregate during storage and should be vigorously vortexed to separate these aggregates.
4. The 2-μm beads (vial C) must be protected from prolonged exposure to light.
5. Do not expose stained CEN or CTN to prolonged light during storage or incubation.
6. Incubation times or temperatures other than those specified may lead to erroneous results.
7. Propidium iodide solution (vial D) must be protected from prolonged exposure to light. Deterioration in stain performance has been observed with short periods of room temperature storage, therefore stained samples must be stored at 2° to 8°C in the dark until analysis (maximum 12 hours).
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