作为一款高端创新型的产品,Cellartis DEF-CS培养系统使得单细胞干细胞操作成为常规的操作。该产品是专门针对人类诱导干细胞(hiPS)和人类胚胎干细胞(hES)研发的高效率增殖用培养基产品体系。该产品系统为all-in-one型,包括了所有组分,客户不需要另外求购组分。该产品是成分确定的培养基产品,而且不需要饲养层。DEF-CS既可以实现单细胞培养,也可以用于传统的iPS培养模式以及大规模干细胞增殖。在使用DEF-CS培养基增殖干细胞时,几乎没有背景分化的问题,这使得细胞筛选不再必需。作为一款创新产品,利用DEF-CS培养干细胞时,可以使用酶消化法(enzymatic passaging)实现需要单细胞操作,这一特点十分有利于高通量细胞鉴别筛选(high-throughput screening)、转染(transfection)、框架接种(scaffold seeding)等。
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■ 产品特点
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· Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation
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■ 产品应用
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· Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold)
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